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Old 12-15-2018, 05:54 AM   #1
bheida
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Location: Hannover

Join Date: Sep 2018
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Default Correct Ratio for two-sided PCR-cleanup with Ampure XP beads

Hey guys,
I want to use Ampure XP beads for size selection after PCR. I tried to find the right ratio of beads : pcr-product but got mixed results so far.

I'm curious, what ratios should work approximately for a two-sided clean-up if i want to select pcr-products around 170-230bp.

I would like to have a very clean product. The PCR is run with genomic DNA which has been sheared to a length of around 10000bp and the Primers used are around 55bp. Both should be cleaned out as well as possible.

Thanks for the input.
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Old 12-16-2018, 12:00 AM   #2
nucacidhunter
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Double SPRI size selection window will be much larger than 50 bp. For your target you need to use Sage Pippin or gel extraction.
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Old 01-10-2019, 02:01 AM   #3
JakobHedegaard
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Take a look at https://ls.beckmancoulter.co.jp/file...SPRIselect.pdf
But I agree with nucacidhunter that 50 bp is a too narrow window for a double-SPRI
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