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Old 01-12-2019, 02:13 AM   #1
pauli.pauli
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Location: Switzerland

Join Date: Jan 2019
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Default Getting protein fasta from RNAseq BAM file (human) paying attention on repeats

Hi everybody,

I have several RNAseq BAM files (mapped with BWA and GATK IndelRealigner) of cancer patients of which I should compare if there any copynumber in repeats appeared (whole transcriptome). I need to find a pipeline to find repeats in with our repeat detection software which needs FASTA and protein sequences as input.

Now I would like to have each patient's personal transcriptome as Fasta format which I could only feed to our software. What do I do now? For example, can I use Blastx, which compares directly to a reference proteome? Does this pay attention to the reading-frame? How do I get a Fasta output with each protein? In this pipeline, it is important to pay special attention to repeats that deviate from the reference.

Many thanks for your help!
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