SEQanswers

Go Back   SEQanswers > Sequencing Technologies/Companies > Illumina/Solexa



Similar Threads
Thread Thread Starter Forum Replies Last Post
HiSeq 4000 upgrade buthercup_ch Illumina/Solexa 4 07-27-2016 03:49 AM
SmartSeq2 on Illumina HiSeq 4000 StephanK Illumina/Solexa 1 07-12-2016 11:44 AM
Mapping rate with PolyA and Ribo-zero selection on NextSeq 500 and HiSeq 4000 Jane M RNA Sequencing 15 05-09-2016 05:15 AM
HiSeq 3000/4000 2x150 data scrosby Illumina/Solexa 10 06-04-2015 09:29 AM
Lotsa new toys from Illumina: HiSeq X Five, 3000, 4000, NextSeq 550 GW_OK Illumina/Solexa 53 05-20-2015 11:30 PM

Reply
 
Thread Tools
Old 09-14-2016, 03:36 AM   #1
sklages
Senior Member
 
Location: Berlin, DE

Join Date: May 2008
Posts: 628
Smile HiSeq 4000 : typical RAW data output in GBytes

Hi,

can someone provide some info on how much raw data (in terms of GBytes, not Gbases) is generated for some typical HiSeq 4000 WGS runs (150,75, whatever)? How much fastq data (GBytes) is generated roughly/typically?

We need to plan computing and storage resources accordingly.

best,
Sven
sklages is offline   Reply With Quote
Old 09-14-2016, 03:21 PM   #2
kcchan
Senior Member
 
Location: USA

Join Date: Jul 2012
Posts: 184
Default

From the Illumina FAQ:

Quote:
The run folder size for the maximum read length of 2 x 150 is about 0.6 TB.
kcchan is offline   Reply With Quote
Old 09-14-2016, 11:16 PM   #3
sklages
Senior Member
 
Location: Berlin, DE

Join Date: May 2008
Posts: 628
Default

Quote:
Originally Posted by kcchan View Post
From the Illumina FAQ:
Sure. For our HiSeq 2000 a few years ago, we had folder sizes up to 4TB; Illumina stated in their FAQ that a run folder is roughly 1TB.

I am just curious how real-world numbers align with Illumina's FAQ.
sklages is offline   Reply With Quote
Old 09-15-2016, 08:23 AM   #4
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,049
Default

Some recent run data folder sizes for HiSeq 4000.

SE 50 bp - 90G
PE 50 bp - 170G
PE 75 bp - 250G
PE 150 bp - 500G
GenoMax is offline   Reply With Quote
Old 09-15-2016, 10:29 PM   #5
sklages
Senior Member
 
Location: Berlin, DE

Join Date: May 2008
Posts: 628
Default

GenoMax, thanks for the numbers. That makes me optimistic :-)
sklages is offline   Reply With Quote
Old 09-16-2016, 01:06 AM   #6
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,049
Default

Should have said that those are raw data folder sizes before bcl2fastq v.2.x conversion.
GenoMax is offline   Reply With Quote
Old 09-16-2016, 01:21 AM   #7
sklages
Senior Member
 
Location: Berlin, DE

Join Date: May 2008
Posts: 628
Default

Quote:
Originally Posted by GenoMax View Post
Should have said that those are raw data folder sizes before bcl2fastq v.2.x conversion.
That's what I was interested in :-)

But btw, what's the fastq output for such runs (roughly)?
sklages is offline   Reply With Quote
Old 09-17-2016, 02:16 AM   #8
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,049
Default

Quote:
Originally Posted by sklages View Post
That's what I was interested in :-)

But btw, what's the fastq output for such runs (roughly)?
Fastq folder size (sample dependent)

PE 50 bp - ~195 G
PE 75 bp - ~275 G
PE 150 bp - ~350-400 G
GenoMax is offline   Reply With Quote
Old 09-17-2016, 09:54 AM   #9
Brian Bushnell
Super Moderator
 
Location: Walnut Creek, CA

Join Date: Jan 2014
Posts: 2,707
Default

Are those numbers for gzipped or raw fastqs?
Brian Bushnell is offline   Reply With Quote
Old 09-17-2016, 07:05 PM   #10
GenoMax
Senior Member
 
Location: East Coast USA

Join Date: Feb 2008
Posts: 7,049
Default

For gzipped fastq's.
GenoMax is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:24 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO