SEQanswers

Go Back   SEQanswers > Literature Watch



Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Functional metagenomics to mine the human gut microbiome for dietary fiber ca Newsbot! Literature Watch 0 09-16-2010 03:30 AM
PubMed: Unlocking short read sequencing for metagenomics. Newsbot! Literature Watch 0 08-03-2010 04:40 AM
PubMed: Comparative metagenomics of Daphnia symbionts. Newsbot! Literature Watch 0 04-23-2009 05:00 AM
PubMed: Methods for comparative metagenomics ECO Metagenomics 0 02-28-2009 01:00 PM
PubMed: Metagenomics: Read length matters. Newsbot! Literature Watch 0 02-13-2008 11:48 AM

Reply
 
Thread Tools
Old 03-25-2011, 06:10 AM   #1
Newsbot!
RSS Posting Maniac
 

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Metagenomics.

Syndicated from PubMed RSS Feeds

Metagenomics.

Methods Mol Biol. 2011;733:173-83

Authors: Gilbert JA, Laverock B, Temperton B, Thomas S, Muhling M, Hughes M

Metagenomics has evolved over the last 3 decades from the analysis of single genes and their apparent diversity in an ecosystem to the provision of complex genetic information relating to whole ecosystems. Metagenomics is a vast subject area in terms of methodology, which encompasses a suite of molecular technologies employed to investigate genomic information from all members of a microbial community. However, the relatively recent developments in high-throughput sequencing platforms have meant that metagenomic can be performed simply by extracting DNA and sequencing it. Here, we outline explicit methodologies for the extraction of metagenomic DNA from marine and sediments/soil environmental samples, the subsequent production and sequencing of large-insert metagenomic libraries, and also shotgun pyrosequencing considerations. We also provide relevant advice on bioinformatic analyses of the complex metagenomic datasets. We hope that the information provided here will be useful to establish the techniques in most reasonably equipped molecular biology laboratories.

PMID: 21431770 [PubMed - in process]



More...
Newsbot! is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 02:13 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO