Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
Comparing detected genes. Coords for probesets of Affy GeneChip Mouse 430 2.0 Array. Azazel Bioinformatics 1 04-11-2011 01:55 AM
PubMed: The use of ultra-dense array CGH analysis for the discovery of micro-copy num Newsbot! Literature Watch 0 01-29-2011 10:50 AM
PubMed: Candidate exome capture identifies mutation of SDCCAG8 as the cause of a reti Newsbot! Literature Watch 0 09-14-2010 10:50 AM
Mutation discovery wrapapu Genomic Resequencing 1 03-24-2010 01:19 AM
PubMed: High definition profiling of mammalian DNA methylation by array capture and s Newsbot! Literature Watch 0 07-08-2009 05:00 AM

Thread Tools
Old 07-25-2009, 05:07 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default PubMed: Mutation discovery in the mouse using genetically guided array capture and re

Syndicated from PubMed RSS Feeds

Mutation discovery in the mouse using genetically guided array capture and resequencing.

Mamm Genome. 2009 Jul 21;

Authors: D'Ascenzo M, Meacham C, Kitzman J, Middle C, Knight J, Winer R, Kukricar M, Richmond T, Albert TJ, Czechanski A, Donahue LR, Affourtit J, Jeddeloh JA, Reinholdt L

Forward genetics (phenotype-driven approaches) remain the primary source for allelic variants in the mouse. Unfortunately, the gap between observable phenotype and causative genotype limits the widespread use of spontaneous and induced mouse mutants. As alternatives to traditional positional cloning and mutation detection approaches, sequence capture and next-generation sequencing technologies can be used to rapidly sequence subsets of the genome. Application of these technologies to mutation detection efforts in the mouse has the potential to significantly reduce the time and resources required for mutation identification by abrogating the need for high-resolution genetic mapping, long-range PCR, and sequencing of individual PCR amplimers. As proof of principle, we used array-based sequence capture and pyrosequencing to sequence an allelic series from the classically defined Kit locus (~200 kb) from each of five noncomplementing Kit mutants (one known allele and four unknown alleles) and have successfully identified and validated a nonsynonymous coding mutation for each allele. These data represent the first documentation and validation that these new technologies can be used to efficiently discover causative mutations. Importantly, these data also provide a specific methodological foundation for the development of large-scale mutation detection efforts in the laboratory mouse.

PMID: 19629596 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 07:36 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO