SEQanswers

Go Back   SEQanswers > General



Similar Threads
Thread Thread Starter Forum Replies Last Post
aligners filtering out reads with excessive read depth? efoss Bioinformatics 2 10-01-2013 11:19 AM
Percent/Fraction/Number of Bases vs. Read Depth plots - How to generate ? medalofhonour Bioinformatics 1 01-14-2013 04:19 AM
Does the read depth distribution(not read depth) matters yuhao Bioinformatics 0 08-09-2012 05:22 AM
Trade off between read length and number of reads ymc RNA Sequencing 3 08-03-2012 02:39 AM
Copy number variation: read depth algorithms and BAF shuteo Bioinformatics 0 07-27-2012 08:19 AM

Reply
 
Thread Tools
Old 02-27-2014, 06:42 AM   #1
angel0071987
Junior Member
 
Location: UK

Join Date: Oct 2011
Posts: 1
Default read depth, number of reads explanation

HI I am very new to sequencing and am trying to learn the basics for use on a miseq sequencer.

i understand that the read length is the number of bases sequenced consectively, but i was hoping that someone can please explain what the number of reads is, and what the depth of sequencing means?

im getting confused as to whether expected read depth’ is the same as ‘times coverage’ of the genome.

I hope you can help.

Thanks
angel0071987 is offline   Reply With Quote
Old 02-27-2014, 07:07 AM   #2
dpryan
Devon Ryan
 
Location: Freiburg, Germany

Join Date: Jul 2011
Posts: 3,480
Default

Crossposted on biostars, where Pierre and I have both replied.
dpryan is offline   Reply With Quote
Reply

Tags
number bases sequenced, read depth

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 03:46 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO