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Old 09-17-2010, 07:11 AM   #1
hongyan
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Location: Canada

Join Date: Sep 2008
Posts: 3
Wink Size selction

I am bothered with one question when I followe the Illumina protocol to prepare the sequence library. I get ChIPed-DNA with the range of 300-600bp using sonicator. Then add adaptors and do size selection of 150-200bp.

If the target protein binds with 600bp ChIPed-DNA, this binding peak will not be detected since only the fragments with the size of 150-200bp are selected for sequence.

What I am thinking is right? I know I do not need to worry about this because every body follows this protocol. I just do not know how and why. Could anybody help me?
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