Go Back   SEQanswers > Literature Watch

Similar Threads
Thread Thread Starter Forum Replies Last Post
PubMed: Massively parallel sequencing, a new method for detecting adventitious agents Newsbot! Literature Watch 1 02-05-2018 05:05 AM
PubMed: Development and implementation of a highly-multiplexed SNP array for genetic Newsbot! Literature Watch 0 07-20-2011 10:30 AM
RNA-Seq: Ribosomal RNA Depletion for Massively Parallel Bacterial RNA-Sequencing Appl Newsbot! Literature Watch 0 03-25-2011 02:00 AM
Spiking phiX into multiplexed libraries as well as barcode sequences cmawhinney Sample Prep / Library Generation 3 09-16-2010 11:13 AM
PubMed: A method for studying protistan diversity using massively parallel sequencing Newsbot! Literature Watch 0 07-28-2009 05:00 AM

Thread Tools
Old 05-13-2010, 02:00 AM   #1
RSS Posting Maniac

Join Date: Feb 2008
Posts: 1,443
Default RNA-Seq: Highly-multiplexed barcode sequencing: an efficient method for parallel anal

Syndicated from PubMed RSS Feeds

Highly-multiplexed barcode sequencing: an efficient method for parallel analysis of pooled samples.

Nucleic Acids Res. 2010 May 11;

Authors: Smith AM, Heisler LE, St Onge RP, Farias-Hesson E, Wallace IM, Bodeau J, Harris AN, Perry KM, Giaever G, Pourmand N, Nislow C

Next-generation sequencing has proven an extremely effective technology for molecular counting applications where the number of sequence reads provides a digital readout for RNA-seq, ChIP-seq, Tn-seq and other applications. The extremely large number of sequence reads that can be obtained per run permits the analysis of increasingly complex samples. For lower complexity samples, however, a point of diminishing returns is reached when the number of counts per sequence results in oversampling with no increase in data quality. A solution to making next-generation sequencing as efficient and affordable as possible involves assaying multiple samples in a single run. Here, we report the successful 96-plexing of complex pools of DNA barcoded yeast mutants and show that such 'Bar-seq' assessment of these samples is comparable with data provided by barcode microarrays, the current benchmark for this application. The cost reduction and increased throughput permitted by highly multiplexed sequencing will greatly expand the scope of chemogenomics assays and, equally importantly, the approach is suitable for other sequence counting applications that could benefit from massive parallelization.

PMID: 20460461 [PubMed - as supplied by publisher]

Newsbot! is offline   Reply With Quote

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 09:55 PM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO