SEQanswers

Go Back   SEQanswers > Applications Forums > Sample Prep / Library Generation



Similar Threads
Thread Thread Starter Forum Replies Last Post
Smart-seq2 sample prep questions PrimerBuffalo Sample Prep / Library Generation 3 11-19-2018 08:08 AM
Help with UMI (unique molecular identifiers) data processing donquijotes Bioinformatics 12 12-01-2017 10:59 PM
Smart-seq2 lowest input? kobeho24 RNA Sequencing 1 06-04-2015 07:19 AM
SMART-seq2 and Nextera XT DNA sample preparation kit wacguy Sample Prep / Library Generation 9 02-09-2015 01:00 AM

Reply
 
Thread Tools
Old 05-11-2017, 01:28 PM   #1
PrimerBuffalo
Junior Member
 
Location: Chicago

Join Date: Mar 2017
Posts: 6
Default UMI in Smart-seq2 protocol

Hi All,

Has anyone had any experience trying to add UMI to the smart-seq2 protocol? I've done some searching, but I haven't found an account of anyone doing so. I would like to know if it is even possible.

Thank you!
PrimerBuffalo is offline   Reply With Quote
Old 05-11-2017, 03:10 PM   #2
nucacidhunter
Jafar Jabbari
 
Location: Melbourne

Join Date: Jan 2013
Posts: 1,232
Default

For this protocol UMI can be added to RT primer and SMART oligo. But they will be lost once amplified cDNA is fragmented/tagmented. With some modifications it is possible to keep primer and SMART oligo UMIs but reads from main body of transcript (except termini) will not have UMI.
nucacidhunter is offline   Reply With Quote
Reply

Tags
cdna synthesis, rna-seq, smart-seq2, umi

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 11:30 AM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2020, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO