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  • samtools flagstat reports an odd number of reads "with itself and mate mapped"

    How can "samtools flagstat" report an odd number of reads "with itself and mate mapped"? I don't get that...

    samtools flagstat reads.bam

    Code:
    161533046 + 0 in total (QC-passed reads + QC-failed reads)
    0 + 0 duplicates
    137350248 + 0 mapped (85.03%:-nan%)
    161533046 + 0 paired in sequencing
    80766523 + 0 read1
    80766523 + 0 read2
    106075436 + 0 properly paired (65.67%:-nan%)
    124869771 + 0 with itself and mate mapped
    12480477 + 0 singletons (7.73%:-nan%)
    8181512 + 0 with mate mapped to a different chr
    4410235 + 0 with mate mapped to a different chr (mapQ>=5)
    Any idea?
    Reads come from a mate pair protocol, sequenced on HiSeq, aligned by bwa.
    Thanks!

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