There is a project which need a referrence sequences, so i collect all the ESTs sequences and four 454 datasets from NCBI and modify to fasta format.
I tried to cluster and asemble with tgicl, but it's failure. My server have only one cpu with 4 core and 4GB RAM.
Who can tell me how to assemble these sequences? more detail more well!
best regards!
I tried to cluster and asemble with tgicl, but it's failure. My server have only one cpu with 4 core and 4GB RAM.
Who can tell me how to assemble these sequences? more detail more well!
best regards!
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