Hello,
We only have a probe sonicator so my chip fragments are peaked around 500bp, ranging from 200~1000bp. The facility guy told me that he would first align the adaptors, then do the PCR (18 cycles), then do the size selection (about 200bp) from gel. My question is that if my DNA fragment centered ~500bp, there must be only a tiny fraction sizing ~100bp which can be later select from the gel. Am I right? The majority of my chip DNA would be discarded. Will this introduce bias?
Thanks a lot! Really do not want to waste my boss' money
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