Hi guys. I have some tissue homogenates in QIAGEN's Buffer RLT+, stored in the -80 freezer. I want to extract some RNA from these samples for downstream NGS applications, and was wondering if it's possible to follow an organic extraction method for samples in RTL+?
I normally homogenise in TRIreagent which is fine, but i can't find the molecular composition of RLT+ anywhere! Is it best to use an RNeasy column or similar to extract my RNA from these homogenates?
Thanks very much
Tali
I normally homogenise in TRIreagent which is fine, but i can't find the molecular composition of RLT+ anywhere! Is it best to use an RNeasy column or similar to extract my RNA from these homogenates?
Thanks very much
Tali