Hi,
when you measure the concentration of your input gDNA or libraries (illumina nextera in my case), do you use the same DNA standards (=kit contents) for both? In my understanding, the standards should have a similar size than the actual sample, which is extremely different between input gDNA and 400pb libraries.
The protocol from Quantifluor mentions this problem (and recommends self-made standards), the protocols for Qubit or Quant-IT don't. Both use lambda DNA (size ~ 48,5 kb) as standards.
How do you handle that in your everyday routine?
best,
Sören
when you measure the concentration of your input gDNA or libraries (illumina nextera in my case), do you use the same DNA standards (=kit contents) for both? In my understanding, the standards should have a similar size than the actual sample, which is extremely different between input gDNA and 400pb libraries.
The protocol from Quantifluor mentions this problem (and recommends self-made standards), the protocols for Qubit or Quant-IT don't. Both use lambda DNA (size ~ 48,5 kb) as standards.
How do you handle that in your everyday routine?
best,
Sören
Comment