Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • unrecognized command mpileup

    Hi,

    I'm using samtools 0.1.7.
    Suddenly I realized that 'mpileup' command is not recognized and when I checked back the mpileup command is not included in the samtools.
    I run this cmd 'samtools mpileup ....' and got error
    [main] unrecognized command 'mpileup'
    Anyone can explain this? Or should i install the latest one?

    Thanks!

  • #2
    Any reason not to upgrade? SAMtools is up to 0.1.13 - I'm guessing mpileup came in after 0.1.7

    Comment


    • #3
      mpileup appears in samtools-0.1.8

      You must upgrade to get mpileup.

      From samtools-0.1.8/NEWS:

      * Added the `mpileup' command which computes the pileup of multiple alignments.

      Comment


      • #4
        i tried to upgrade the samtools 0.1.13 but still couldnt find the mpileup.
        i'm lost right now to install samtools eventhough i read the installation manual.
        can u guys assist me on samtools installation .
        may be i miss something during installation.
        thanks!

        Comment


        • #5
          Quite straightforward. Install subversion (svn) and do

          Code:
          svn co https://samtools.svn.sourceforge.net/svnroot/samtools/trunk/samtools
          A folder named 'samtools' will be created. Every time a new version is released cd to that directory and run

          Code:
          $ svn update
          $ make
          You can then copy the latest samtools binary to your ${PATH}

          Comment


          • #6
            thanks all.
            btw, success to install samtools.
            my mistaked because there is missing library.
            i checked back the library and install.
            then samtools is ok to use

            Comment


            • #7
              would you like to tell me how do you done it successfully? I have the same error!
              Thanks!
              Best wishes!

              Comment


              • #8
                hi qqtwee,


                i can't remember as it happen long time ago. but the key is u need to check whether u have the complete library that needed by Samtools to be succesfully installed.
                try to read the error log. could be missing some of library from your computer.

                good luck!

                Comment

                Latest Articles

                Collapse

                • seqadmin
                  Current Approaches to Protein Sequencing
                  by seqadmin


                  Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
                  04-04-2024, 04:25 PM
                • seqadmin
                  Strategies for Sequencing Challenging Samples
                  by seqadmin


                  Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
                  03-22-2024, 06:39 AM

                ad_right_rmr

                Collapse

                News

                Collapse

                Topics Statistics Last Post
                Started by seqadmin, 04-11-2024, 12:08 PM
                0 responses
                24 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 04-10-2024, 10:19 PM
                0 responses
                25 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 04-10-2024, 09:21 AM
                0 responses
                21 views
                0 likes
                Last Post seqadmin  
                Started by seqadmin, 04-04-2024, 09:00 AM
                0 responses
                52 views
                0 likes
                Last Post seqadmin  
                Working...
                X