Hi,
We have done many RNA seq experiments, but this will be our small RNA seq experiment. We are following small RNA library preparation using Total RNA sequencing kit. The protocol suggests either using total RNA or enriched small RNA for the library preparation. For the enrichment protocol we do not have sufficient total RNA amount (<2ug). So other option is to start the library prep with total RNA. Has anyone prepared small RNA library using total RNA as input. Can anyone suggest which is the best way to go - enrich small RNA with the available total RNA or use total RNA as input for library prep.
Please advice
We have done many RNA seq experiments, but this will be our small RNA seq experiment. We are following small RNA library preparation using Total RNA sequencing kit. The protocol suggests either using total RNA or enriched small RNA for the library preparation. For the enrichment protocol we do not have sufficient total RNA amount (<2ug). So other option is to start the library prep with total RNA. Has anyone prepared small RNA library using total RNA as input. Can anyone suggest which is the best way to go - enrich small RNA with the available total RNA or use total RNA as input for library prep.
Please advice
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