Tweet
I am having trouble size selecting on the TBE gels for the sm RNA preps? Any advice on what dyes and stains work best?
-
-
staining TBE gel
We use SYBR for staining miRNA libraries post PCR. Our buffers gel loading buffers come from a kit. Resolution and separation of miRNAs products works well with SYBR. -
what percentage gel works best? did you use a TBE-Urea gel?Comment
-
8% no ureaComment
-
i was under the impression it is standard to use denaturing conditions when running RNA?Comment
-
Hi
Not sure what are you asking for...isolating small RNAs or libraries made from miRNAs?
At least, I did it (small RNA isolation) with 15% denaturing (UreaGel from National Diagnostics) PAGE.
The library purification (eliminate any kind of primer dimer, adapters, etc) I used 10% non-denatuting PAGE.Comment
-
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
Comment