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Dear all,
My samples quantities was 246, 184, 112, 133 and 232 ng. I pooled them all and run them in a gel for site selection. Afterward I did purified them using columns. Surprisingly and after ,measuring them with Qubit their quantity became 10.7 ng.
First, I would like to know why they have such a low concentration?
second, if the beads are the reason, then how can I avoid this?
Third, will these samples still work to proceed with? for Ion one touch?
My samples quantities was 246, 184, 112, 133 and 232 ng. I pooled them all and run them in a gel for site selection. Afterward I did purified them using columns. Surprisingly and after ,measuring them with Qubit their quantity became 10.7 ng.
First, I would like to know why they have such a low concentration?
second, if the beads are the reason, then how can I avoid this?
Third, will these samples still work to proceed with? for Ion one touch?