batch tool for checking primers

nouse · 02-14-2017, 06:46 AM

Hi, i am about to order 400 barcoded primers (100 barcodes + 2 directions x 2 Genes of Interest).

Usually i check my primers with the OligoAnalyzer by IDT, but they dont accept the upload of fasta files or so.

My question is if there is a tool which can assess all 400 primers and can give out: Tm, worst Hairpin/selfdimer/crossdimer deltaGs, at my desired PCR parameters?

That would be handy. Thank you!

/edit: I just came across barcrawl. Is it working the way as described above?

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02-14-2017, 06:46 AM	#1
nouse Member Location: Germany Join Date: Sep 2013 Posts: 11	batch tool for checking primers Hi, i am about to order 400 barcoded primers (100 barcodes + 2 directions x 2 Genes of Interest). Usually i check my primers with the OligoAnalyzer by IDT, but they dont accept the upload of fasta files or so. My question is if there is a tool which can assess all 400 primers and can give out: Tm, worst Hairpin/selfdimer/crossdimer deltaGs, at my desired PCR parameters? That would be handy. Thank you! /edit: I just came across barcrawl. Is it working the way as described above? Last edited by nouse; 02-14-2017 at 06:48 AM.