As dumb as this sounds, can someone please tell me if PE primers can work with SE adapters to successfully generate PE data? We suspect this combination may have occurred on 3 occasions but each time there was a different outcome: only read 1 worked (GAII, long time ago), both reads generated clusters and went through the pipeline without errors, and no clusters at all (latter 2 on GAIIx, SCS2.5).
I checked some helpful diagrams provided by this group and it looks like it should not have worked at all. But I cannot explain our 3 different results, so any help will be greatly appreciated.
I checked some helpful diagrams provided by this group and it looks like it should not have worked at all. But I cannot explain our 3 different results, so any help will be greatly appreciated.
Comment