Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • breakdancer several bam misunderstand

    Hello,

    I am calling breakdancer to see which structural variants are different from one sample to another. For these I do this two steps:
    perl perl/bam2cfg.pl -g -h ivia_040_10.bam ivia_214_10.bam > both.cfg
    breakdancer_max -g bed both.cfg > both.sv

    My problem is that breakdancer is not reporting well the sv, for example, I see the same sv in two different lines,line 2 and 3, in the file both.sv, it just changes two bases, and if you see it in IGV, is a perfect deletion in both samples ex:

    1. scaffold_1 348436 88+2- scaffold_1 349316 1+166- DEL 630 99 87 ivia_040_10.bam|84:ivia_214_10.bam|3 NA 0.30
    2. scaffold_1 355952 122+0- scaffold_1 356266 1+247- DEL 329 99 122 ivia_040_10.bam|122 0.01 6.48
    3. scaffold_1 355954 122+1- scaffold_1 356266 0+124- DEL 333 99 122 ivia_214_10.bam|122 NA NA
    4. scaffold_1 360645 36+35- scaffold_1 361319 36+35- INS -200 99 35 ivia_040_10.bam|20:ivia_214_10.bam|15 NA NA

    The thing is that some of them are well detected, like in line 1 or 4.

    could anyone help me with this?

    Thanks!

Latest Articles

Collapse

  • seqadmin
    Recent Advances in Sequencing Analysis Tools
    by seqadmin


    The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...
    05-06-2024, 07:48 AM
  • seqadmin
    Essential Discoveries and Tools in Epitranscriptomics
    by seqadmin




    The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
    04-22-2024, 07:01 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by seqadmin, 05-07-2024, 06:57 AM
0 responses
12 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-06-2024, 07:17 AM
0 responses
16 views
0 likes
Last Post seqadmin  
Started by seqadmin, 05-02-2024, 08:06 AM
0 responses
22 views
0 likes
Last Post seqadmin  
Started by seqadmin, 04-30-2024, 12:17 PM
0 responses
24 views
0 likes
Last Post seqadmin  
Working...
X