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T over-representation in NextSeq500 run
Hello, we got strange results from some of the recent RNA-seq runs on NextSeq500; example: 
Funny is that we have observed it in some runs while other runs from the same experiment (all processed with NextFlex kit and after polyA selection) but sequenced later worked fine. (RIN was ok, no weird yield after library preparation)
Illumina blaims the sample... What makes sense as the re-run with flowcell from different batch showed same result. But I do not understand what would make such a strange result.
Any thoughts?
Funny is that we have observed it in some runs while other runs from the same experiment (all processed with NextFlex kit and after polyA selection) but sequenced later worked fine. (RIN was ok, no weird yield after library preparation)
Illumina blaims the sample... What makes sense as the re-run with flowcell from different batch showed same result. But I do not understand what would make such a strange result.
Any thoughts?