Hi ,
We have performed Chromatin immunoprecipitation experiments.
Our goal is to find out the genome-wide bindings of a transcription factor so we sent our ChIP-qPCR validated sample to do the illumina sequencing.
Finally we were frustrated by the ChIP-seq results which turn out to be random reads mapped to genome.
What's wrong with the ChIP-seq? Is it common or possible that ChIP-qPCR doesn't fit ChIP-seq?
We have performed Chromatin immunoprecipitation experiments.
Our goal is to find out the genome-wide bindings of a transcription factor so we sent our ChIP-qPCR validated sample to do the illumina sequencing.
Finally we were frustrated by the ChIP-seq results which turn out to be random reads mapped to genome.
What's wrong with the ChIP-seq? Is it common or possible that ChIP-qPCR doesn't fit ChIP-seq?
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