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Old 12-10-2012, 12:50 PM   #1
ngseq
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Location: Philadelphia, PA

Join Date: Mar 2010
Posts: 19
Smile duplicate reads removal for amplicon libraries?

Hi everyone,

Do you revove duplicate reads during data analysis if the library is an amplicon library or a raindance-captured library. My understanding is that there may seem to be a lot of "duplicates" in the reads but they may not truely be duplicates as the molecules may have been amplified from different template despite they share the same ends.

Any thoughts are appreciated. Thanks!
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