Hello-
So I am working on replicating an analysis from a published study that used 454 sequencing with titanium chemistry, and have downloaded all sequences from the SRA database. Here is an example sequence:
And here is the corresponding barcode/primer sequence sent to me from from the author. barcode bolded, primer underlined:
ACGAGTGCGTTCCTSCGCTTATTGATATGC
Both of these are in this sequence, from the sample:
However, there is a 15bp sequence preceding the barcode/primer: GACTACACGTAGTAT
I don't know what this is, but it causes my split libraries commands to fail in QIIME. I've seen that some samples in this data set have this identical sequence preceding them, while others have a slightly different sequence but its still 15bp. Any idea what this might be? Is it an artifact of 454 Titanium?
So I am working on replicating an analysis from a published study that used 454 sequencing with titanium chemistry, and have downloaded all sequences from the SRA database. Here is an example sequence:
Code:
GACTACACGTAGTATACGAGTGCGTTCCTGCGCTTATTGATATGCTTAAGTTCAGCGGGTATCCCTACCTGATCCGAGGTCAAAGTAAAAGGCTTGTGGAATGGAGTCTGGCTGAAATCGCTTGCAATGTGCTGCGCACGAAGCCAACATACCGGCTGCCAATGAATTTGAGGCGAGTCCACGCGCTGAGGCGGAACAAACACCCAACACCAAGCATAGCTTGAAGGTTTAAATGACGCTCGAACAGGCATGCCCAACGGAATACCGAAGGGCAATACTAGGTGTGGTCGGCGTCTCTCAAGGCACACAGGGGATAGGNNN
ACGAGTGCGTTCCTSCGCTTATTGATATGC
Both of these are in this sequence, from the sample:
Code:
GACTACACGTAGTAT[B]ACGAGTGCGT[/B][U]TCCTGCGCTTATTGATATGC[/U]TTAAGTTCAGCGGGTATCCCTACCTGATCCGAGGTCAAAGTAAAAGGCTTGTGGAATGGAGTCTGGCTGAAATCGCTTGCAATGTGCTGCGCACGAAGCCAACATACCGGCTGCCAATGAATTTGAGGCGAGTCCACGCGCTGAGGCGGAACAAACACCCAACACCAAGCATAGCTTGAAGGTTTAAATGACGCTCGAACAGGCATGCCCAACGGAATACCGAAGGGCAATACTAGGTGTGGTCGGCGTCTCTCAAGGCACACAGGGGATAGGNNN
However, there is a 15bp sequence preceding the barcode/primer: GACTACACGTAGTAT
I don't know what this is, but it causes my split libraries commands to fail in QIIME. I've seen that some samples in this data set have this identical sequence preceding them, while others have a slightly different sequence but its still 15bp. Any idea what this might be? Is it an artifact of 454 Titanium?
Comment