Hi everyone,
I want to analyse my .bam files (sorted and indexed), that have been mapped by someone else before.
These are as this:
- sample.merged.mdup.forward.bam
- sample.merged.mdup.reverse.bam
when looking for ht-seq counts tutorials, inputs were only one bam (reverse+forward) and the gff.
#1 What I wanted to know is : is it possible to use the sample.merged.mdup.forward.bam
and the sample.merged.mdup.reverse, as my inputs (+gff)?
#2 If yes, how does the script line looks (i'm beginner in computational analysis :/ )
#3 If not, does it need to merge the forward and the reverse together?
#4 What does "mdup" mean?
Many thanks in advance
I want to analyse my .bam files (sorted and indexed), that have been mapped by someone else before.
These are as this:
- sample.merged.mdup.forward.bam
- sample.merged.mdup.reverse.bam
when looking for ht-seq counts tutorials, inputs were only one bam (reverse+forward) and the gff.
#1 What I wanted to know is : is it possible to use the sample.merged.mdup.forward.bam
and the sample.merged.mdup.reverse, as my inputs (+gff)?
#2 If yes, how does the script line looks (i'm beginner in computational analysis :/ )
#3 If not, does it need to merge the forward and the reverse together?
#4 What does "mdup" mean?
Many thanks in advance