Just curious!? At what point does the number of clusters max out the ability of the machine to read it well (from first base report) - on both a HiSeq or GA flowcell? Thanks
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For the HiSeq, I have gotten great results when I aim for the 700,000 K/mm^2 cluster density off of the first base report (usually about 90-120 million reads pass filter). I have had some luck with shooting as high as 1,000,000 K/mm^2, but it's hit or miss and I wouldn't recommend it.
For the GAIIx, I usually aim for 350,000 clusters/mm^2 off of the first base report and about 35-40 million reads pass filter.
Hope this helps!
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Originally posted by kthai View PostFor the HiSeq, I have gotten great results when I aim for the 700,000 K/mm^2 cluster density off of the first base report (usually about 90-120 million reads pass filter). I have had some luck with shooting as high as 1,000,000 K/mm^2, but it's hit or miss and I wouldn't recommend it.
For the GAIIx, I usually aim for 350,000 clusters/mm^2 off of the first base report and about 35-40 million reads pass filter.
Hope this helps!
Out of curiosity, what concentration are you loading on the GAIIx?
Best Regards.
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