Hi everyone,
We are planning to treat total RNA (rRNA removed) with bisulfite and convert the nonmethylated cytosines to uracil. This is a harsh treatment and will brake up mRNA into very small fragments.
We will then use the TruSeq small RNA kit to produce the library. Thus we are expecting to be able to sequence all sorts of small RNAs, together with the chopped up fragments of mRNA.
Has anybody done bisulfite sequencing, and is able to provide advice or warn me of any pitfalls?
Thanks a lot, I appreciate any comment.
Maike
We are planning to treat total RNA (rRNA removed) with bisulfite and convert the nonmethylated cytosines to uracil. This is a harsh treatment and will brake up mRNA into very small fragments.
We will then use the TruSeq small RNA kit to produce the library. Thus we are expecting to be able to sequence all sorts of small RNAs, together with the chopped up fragments of mRNA.
Has anybody done bisulfite sequencing, and is able to provide advice or warn me of any pitfalls?
Thanks a lot, I appreciate any comment.
Maike
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