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I've been trimming paired reads with DynamicTrim based on phred scores and using LengthSort to separate out those under 15 bases. LengthSort separates them into 4 files: 2 for the good reads, one for those under 15 bases, and one for the mates of the reads that were under 15 bases (but which are themselves fine). Is there any way I can still use the reads who were unfortunate enough to have a <15 base mate, or are they complete junk since they no longer have a mate? I am currently using bwa for alignment
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The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist on Modified Bases...Yesterday, 07:01 AM -
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...04-04-2024, 04:25 PM
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