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Old 08-27-2019, 11:11 PM   #1
SapirP
Junior Member
 
Location: Israel

Join Date: Aug 2019
Posts: 3
Default UMI-tools running error- help needed :)

Hi everyone,

I am trying to work with UMI-tools. After installation, I tried to follow the example they provide.

After executing this command:

umi_tools extract --stdin=example.fastq.gz --bc-pattern=NNNNNNNNN --log=processed.log --stdout processed.fastq.gz

I encountered this error:

Traceback (most recent call last):
File "/usr/local/bin/umi_tools", line 5, in <module>
from pkg_resources import load_entry_point
File "<frozen importlib._bootstrap>", line 2237, in _find_and_load
File "<frozen importlib._bootstrap>", line 2226, in _find_and_load_unlocked
File "<frozen importlib._bootstrap>", line 1191, in _load_unlocked
File "<frozen importlib._bootstrap>", line 1161, in _load_backward_compatible
File "/usr/local/lib/python3.4/dist-packages/setuptools-10.0-py3.4.egg/pkg_resources/__init__.py", line 2957, in <module>
File "/usr/local/lib/python3.4/dist-packages/setuptools-10.0-py3.4.egg/pkg_resources/__init__.py", line 567, in _build_master
File "/usr/local/lib/python3.4/dist-packages/setuptools-10.0-py3.4.egg/pkg_resources/__init__.py", line 876, in require
File "/usr/local/lib/python3.4/dist-packages/setuptools-10.0-py3.4.egg/pkg_resources/__init__.py", line 761, in resolve
pkg_resources.DistributionNotFound: matplotlib

Nevertheless, matplotlib is installed on our server, and according to pip it is updated. Did anyone else encountered this problem or has any ideas for a solution?

Thank you so much.
Best,
Sapir
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Old 09-04-2019, 05:57 AM   #2
SapirP
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Location: Israel

Join Date: Aug 2019
Posts: 3
Default

Problem solved.
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matplotlib, programs;bioinformatic, python, umi-tools, unix

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