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  • Using the NEXTflex Small RNA-Seq Kit v3 for RIP-Seq, CLIP-Seq and Ribosomal Profiling

    A number of our customers have asked for a protocol which would enable them to use the NEXTflex™ Small RNA Seq Kit v3 for ribosomal profiling, RIP-Seq (RNA binding protein immunoprecipitation and sequencing), and CLIP-Seq (cross-linking immunoprecipitation and sequencing) experiments. To use the NEXTflex Small RNA-Seq Kit V3, the RNA of interest must have a 5' monophosphate and 3' hydroxyl. Using the following steps posted on our website, these modifications can easily be added to most RNA molecules with T4 polynucleotide kinase (PNK).

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  • seqadmin
    Strategies for Sequencing Challenging Samples
    by seqadmin


    Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...
    03-22-2024, 06:39 AM
  • seqadmin
    Techniques and Challenges in Conservation Genomics
    by seqadmin



    The field of conservation genomics centers on applying genomics technologies in support of conservation efforts and the preservation of biodiversity. This article features interviews with two researchers who showcase their innovative work and highlight the current state and future of conservation genomics.

    Avian Conservation
    Matthew DeSaix, a recent doctoral graduate from Kristen Ruegg’s lab at The University of Colorado, shared that most of his research...
    03-08-2024, 10:41 AM

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