Hi everyone!
I am currently looking at cDNA expression. However, I don't understand the concept of depth/coverage. Some people say that it is how many times a nucleotide is sequenced. I thought that the greater the depth, the bigger the sequenced cDNA...
Finally, assuming it is the former, if we say a coverage of 20x, do we mean that we have sequenced that nucleotide in 20 independent runs? Moreover, is each run made with a different sample?
Thank you for your help.
I am currently looking at cDNA expression. However, I don't understand the concept of depth/coverage. Some people say that it is how many times a nucleotide is sequenced. I thought that the greater the depth, the bigger the sequenced cDNA...
Finally, assuming it is the former, if we say a coverage of 20x, do we mean that we have sequenced that nucleotide in 20 independent runs? Moreover, is each run made with a different sample?
Thank you for your help.
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