Hello,
For financial reasons my lab could not use replicates for the 2 conditions of my RNA seq experiment... I am a grad student . I performed a differential expression analysis using Trinity with EdgeR (the built-in command).. I did get a subset of differentially expressed contigs. Does anyone know how Trinity/edgeR calls these particular contigs as differentially expressed when there are no replicates? The commands are all pre-set, so I'm not sure what is going on there.
For financial reasons my lab could not use replicates for the 2 conditions of my RNA seq experiment... I am a grad student . I performed a differential expression analysis using Trinity with EdgeR (the built-in command).. I did get a subset of differentially expressed contigs. Does anyone know how Trinity/edgeR calls these particular contigs as differentially expressed when there are no replicates? The commands are all pre-set, so I'm not sure what is going on there.
Comment