Can anyone help me with coded/tagged primers to be used with 454 sequencing, please?
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We constructed error-correcting DNA barcodes that allow one run of a massively parallel pyrosequencer to process up to 1,544 samples simultaneously. Using these barcodes we processed bacterial 16S rRNA gene sequences representing microbial communities in 286 environmental samples, corrected 92% of s …
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Barcoded primers
Hi ECO, thanks for the reply. We have just started with the 454 high-throughput amplicon sequencing. I read the paper by Hamady et al., 2008. Results look good; they were able to correct decoding errors in 1,241 out of 1,345 sequences. But, felt a bit difficult to follow as I have never used error-correcting codes before. Do you have any previous experience in using error-correcting codes?
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