Hello folks,
Currently I'm looking for cost effective approaches to cover intronic and intergenic SNPs variants using Illumina NGS workflow? Some targeted enrichment protocol address that issue (i.e. TSCancer) and include probes for selected SNPs (~240), however I wonder is it possible to capture intronic variants using whole exome analysis? The conventional genotyping arrays would be the answer, however personally I consider this methodology outdated and looking for applicable alternative with NGS (whole genome seq would be ideal, however it's still too costly). Are there any protocols to customise exome enrichment kits and add desired non-exonic variants content?
thank you in advance for help,
R.
Currently I'm looking for cost effective approaches to cover intronic and intergenic SNPs variants using Illumina NGS workflow? Some targeted enrichment protocol address that issue (i.e. TSCancer) and include probes for selected SNPs (~240), however I wonder is it possible to capture intronic variants using whole exome analysis? The conventional genotyping arrays would be the answer, however personally I consider this methodology outdated and looking for applicable alternative with NGS (whole genome seq would be ideal, however it's still too costly). Are there any protocols to customise exome enrichment kits and add desired non-exonic variants content?
thank you in advance for help,
R.
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