NEB is selling 2 kits to prepare 454 libraries using dA-tailed ligation:
1. NEBNext Quick DNA Sample Prep Master Mix Set 2
2. NEBNext Quick DNA Sample Prep Reagent Set 2
In the first kit they give the actual enzyme concentrations in the mix, except for the Quick ligase. To decrease the price in preparing 20 libraries, I want to order the NEB enzymes separately. Does anyone have experience in doing it and how much of the Quick ligase (NEB cat. n. M2200S) you use?
Thanks.
1. NEBNext Quick DNA Sample Prep Master Mix Set 2
2. NEBNext Quick DNA Sample Prep Reagent Set 2
In the first kit they give the actual enzyme concentrations in the mix, except for the Quick ligase. To decrease the price in preparing 20 libraries, I want to order the NEB enzymes separately. Does anyone have experience in doing it and how much of the Quick ligase (NEB cat. n. M2200S) you use?
Thanks.