Hi!
I want to quantify mRNA libraries produced with the TruSeq RNA Sample Preparation Kit from Illumina.
I have used Bioanalyzer, Qubit and qPCR (Kapa library quantification kit) and got, as expected, very different results. Especially between qPCR and the other two methods.
I have attached the bioanalyzer file of the libraries as well as word.doc that lists the molarities of the analyzed libraries.
Any comments from experienced lib prep people? Which meassurement should I trust? Are Bioanalyzer and Qbit usually underestimating the DNA concentration? Is this an expected result?
Any comments are welcome!
I want to quantify mRNA libraries produced with the TruSeq RNA Sample Preparation Kit from Illumina.
I have used Bioanalyzer, Qubit and qPCR (Kapa library quantification kit) and got, as expected, very different results. Especially between qPCR and the other two methods.
I have attached the bioanalyzer file of the libraries as well as word.doc that lists the molarities of the analyzed libraries.
Any comments from experienced lib prep people? Which meassurement should I trust? Are Bioanalyzer and Qbit usually underestimating the DNA concentration? Is this an expected result?
Any comments are welcome!
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