Go Back   SEQanswers > Bioinformatics > Bioinformatics

Similar Threads
Thread Thread Starter Forum Replies Last Post
Galaxy smallRNA Analysis help smallSeq Bioinformatics 2 02-25-2016 03:55 PM
smallRNA annotation NicoBxl Bioinformatics 8 07-18-2012 09:11 AM
smallRNA data dnusol Bioinformatics 1 02-21-2011 11:38 PM
SmallRNA analysis pipeline naluru SOLiD 5 12-12-2010 11:59 PM
multiplexing smallRNA moguin Illumina/Solexa 7 09-16-2010 02:06 PM

Thread Tools
Old 03-06-2017, 01:04 AM   #1
Location: us

Join Date: Apr 2016
Posts: 14
Default smallRNA: how analyze

I am new to smallRNA seq and I am a bit confused as to analyze my data (from human genome).
it is better to:
1) align against ncRNA transcriptome from Ensembl
2) align against genome
3) aling against miRNA transcript, take the unaligned reads, align them against genome and after that annotate them with gtf file.

Or are there better options?

Thank you in advance
edeka is offline   Reply With Quote
Old 03-08-2017, 10:16 PM   #2
Location: Santa Cruz, CA

Join Date: Feb 2017
Posts: 17

I found that when exploring new data CAP-miRSeq is really helpful:
sbarberan is offline   Reply With Quote


Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off

All times are GMT -8. The time now is 12:34 AM.

Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2021, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO