SEQanswers

Go Back   SEQanswers > General



Similar Threads
Thread Thread Starter Forum Replies Last Post
Question About Illumina Paired end Sequencing Dr. Hybe Illumina/Solexa 10 06-30-2011 06:51 AM
how much DNA do I need for paired-end sequencing? Simone78 Sample Prep / Library Generation 6 03-17-2011 09:11 AM
which alignment tool allows to anchor pair end reads over repeats? Inti Bioinformatics 2 11-17-2010 01:48 PM
Paired End Sequencing bulletproofpenguin Bioinformatics 2 03-23-2010 07:39 PM
RNA-seq paired-end sequencing djm2007 Bioinformatics 0 01-05-2010 11:16 AM

Reply
 
Thread Tools
Old 10-15-2008, 05:06 AM   #1
bbjdy
Junior Member
 
Location: Belgium

Join Date: Oct 2008
Posts: 3
Default paired end sequencing - direct/incerted repeats

Hi all,

Doe anyone have experience with nextgen sequencing of BACs containing large direct and inverted repeats? Which technology (454 or solexa, using resp. emulsion-PCR on beads and brigde-PCR on solid phase) would be most suitable for this?
how long can you go for a paired end library on 454 or Solexa (I know with 454 there is a kit for 3K long-tag paired end sequencing)? And what is the accuracy of library length (e.g. 3 Kb +- 100? bp)?
Any help on this is welcome! Thanks,

Jurgen
bbjdy is offline   Reply With Quote
Old 01-01-2009, 02:16 PM   #2
mchaisso
Member
 
Location: Seattle, WA

Join Date: Apr 2008
Posts: 84
Default

One possible approach if the coverage is high enough is to simply look for breakpoints that are sequenced (repeat junctions, etc).
mchaisso is offline   Reply With Quote
Reply

Thread Tools

Posting Rules
You may not post new threads
You may not post replies
You may not post attachments
You may not edit your posts

BB code is On
Smilies are On
[IMG] code is On
HTML code is Off




All times are GMT -8. The time now is 05:21 PM.


Powered by vBulletin® Version 3.8.9
Copyright ©2000 - 2018, vBulletin Solutions, Inc.
Single Sign On provided by vBSSO