We have been preparing libraries for Illumina GA and we have some problems with RNA-seq libraries. The issue that we are having is that we are seeing two peaks in bioanalyzer data (attached ppt). We are seeing this in all our RNA seq samples - no matter what organism used. We are using Illumina RNAseq kit. have anyone seen something like this? I would really appreciate any advice.
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Originally posted by zorph View Posthello
has anyone tried to do two rounds of DSN normalization (specifically for RNA-seq)? If so, how did you do it and how did it go? Thanks.
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We are also seeing double peaks in our RNA-seq library preps, even though all samples have been gel extracted at 350bp. Does anyone know how it is possible to still have 700 bp fragments after gel extraction and what we can do to remove the longer fragments? Thanks.
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