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  • Simulation of a small RNA-seq experiment

    Hi, I would like to know if it is possible to simulate a small RNA-seq dataset because I found tools only for RNA seq.
    Thank you.

  • #2
    Perhaps you could clarify your goal?

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    • #3
      I would like to have a synthetic small RNA-seq for validation purposes.

      Comment


      • #4
        That's pretty vague. Since you are not stating any specific goals or metrics, I recommend you download some real small-RNA data from somewhere (such as SRA) and use that for validation. If you are not trying to measure anything in particular, real data is always better than synthetic data.
        Last edited by Brian Bushnell; 07-13-2016, 07:23 PM.

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        • #5
          Are you looking for small (as in amount of reads/data) or small as in small RNA-seq data?

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          • #6
            Small as in small RNA-seq data, like a real small rna sequencing for miRNAs.

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            • #7
              I don't know of a simulation program that can generate smallRNA seq data. Can you use a real dataset from here?
              Last edited by GenoMax; 07-14-2016, 05:26 AM.

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              • #8
                Thank you.

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