Hi everyone
Recently received my sequences back from an Illumina MiSeq 16S V4 300bp PE run, performed by a biotech company. The DNA was extracted from the gut of fish.
Although I received many millions of reads (approx. 1m raw reads/sample), after running the samples through Mothur, there seems to be a dominance of one particular otu, up to 99% in the case of some samples.
I am wondering whether anybody else experiences similar patterns? I know each study is different depending on the environment, but I am just wondering if the pattern of one otu totally dominating is a common one with these types of NGS studies?
Thank you
Egansbay
Recently received my sequences back from an Illumina MiSeq 16S V4 300bp PE run, performed by a biotech company. The DNA was extracted from the gut of fish.
Although I received many millions of reads (approx. 1m raw reads/sample), after running the samples through Mothur, there seems to be a dominance of one particular otu, up to 99% in the case of some samples.
I am wondering whether anybody else experiences similar patterns? I know each study is different depending on the environment, but I am just wondering if the pattern of one otu totally dominating is a common one with these types of NGS studies?
Thank you
Egansbay
Comment