PubMed: Accurate determination of microbial diversity from 454 pyrosequencing data.

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Related Articles Accurate determination of microbial diversity from 454 pyrosequencing data.

Nat Methods. 2009 Aug 9;

Authors: Quince C, Lanzén A, Curtis TP, Davenport RJ, Hall N, Head IM, Read LF, Sloan WT

We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated.

PMID: 19668203 [PubMed - as supplied by publisher]

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08-12-2009, 05:00 AM	#1
Newsbot! RSS Posting Maniac Join Date: Feb 2008 Posts: 1,443	PubMed: Accurate determination of microbial diversity from 454 pyrosequencing data. Syndicated from PubMed RSS Feeds Related Articles Accurate determination of microbial diversity from 454 pyrosequencing data. Nat Methods. 2009 Aug 9; Authors: Quince C, Lanzén A, Curtis TP, Davenport RJ, Hall N, Head IM, Read LF, Sloan WT We present an algorithm, PyroNoise, that clusters the flowgrams of 454 pyrosequencing reads using a distance measure that models sequencing noise. This infers the true sequences in a collection of amplicons. We pyrosequenced a known mixture of microbial 16S rDNA sequences extracted from a lake and found that without noise reduction the number of operational taxonomic units is overestimated but using PyroNoise it can be accurately calculated. PMID: 19668203 [PubMed - as supplied by publisher] More...