Hello all,
Using BLAST, I am trying to identify thousands of sequences. To make my job a bit easier, I would like to classify these sequences first by sorting them on phylum e.g. only keep BLAST hits from a certain pylum. To do this, I need to generate het complete linage of the hit using the NCBI taxonomic database. I know you can query the database with biopython. over the internet, but the machine I am working on has no internet access.
Could anyone give me some insight into this?
Using BLAST, I am trying to identify thousands of sequences. To make my job a bit easier, I would like to classify these sequences first by sorting them on phylum e.g. only keep BLAST hits from a certain pylum. To do this, I need to generate het complete linage of the hit using the NCBI taxonomic database. I know you can query the database with biopython. over the internet, but the machine I am working on has no internet access.
Could anyone give me some insight into this?