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  • TruSight One - Hiseq Loading Concentration

    Hello,

    I had a quick look through the pre-existing threads but couldn't find an answer so forgive me if it's already been answered!

    I'm going to be loading a TruSight One library on the Hiseq 2500 but the protocol doesn't tell me the pM concentration to load

    I could just try 12pM and adjust it according to the result but I'd like to avoid too much trial and error (mainly due to £££!)

    Has anybody successfully run this library on the Hiseq & would you mind telling me the concentration of library you've been running and the cluster density you obtained?

    Thank you in advance

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