Hello,
I am very new to sequencing and de novo assemblies. I am using CLC Genomics Workbench and am importing my fastq files.
It is asking for paired end minimum distance and maximum distance. I do not know how to calculate that.
Here is my information:
The Machine was the Illumina MiSeq.
The quality score selected, Illumina 1.8.
-----------(Read1)-----------250bp---------------------> gap <----------------------250bp---------(Read2)------------
My library size was 960, which breaks down as follows:
[ Illumina Specific Adapters, 60bp ]----------------------250bp---------------------> gap (~340bp) <----------------------250bp---------------------[ Illumina Specific Adapters, 60bp ]
Thank you for your time
I am very new to sequencing and de novo assemblies. I am using CLC Genomics Workbench and am importing my fastq files.
It is asking for paired end minimum distance and maximum distance. I do not know how to calculate that.
Here is my information:
The Machine was the Illumina MiSeq.
The quality score selected, Illumina 1.8.
-----------(Read1)-----------250bp---------------------> gap <----------------------250bp---------(Read2)------------
My library size was 960, which breaks down as follows:
[ Illumina Specific Adapters, 60bp ]----------------------250bp---------------------> gap (~340bp) <----------------------250bp---------------------[ Illumina Specific Adapters, 60bp ]
Thank you for your time
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