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08-07-2020, 08:57 AM
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#1 |
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Junior Member
Location: Sevilla, Spain Join Date: Aug 2020
Posts: 2
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Is a compositional bias in Sequence Content of initial bases in WES reads normal?
Per Base Sequence Content
Any experienced Whole Exome Sequencing analyst/experimentalist: the Per Base Sequence Content of the initial couple of bases from the 3' end of my WES reads have some type of compositional bias. Is this some "harmless" procedural or technical bias occurring during sequencing or is it something to be worried about for the downstream analysis? 
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08-07-2020, 12:20 PM
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#2 |
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Senior Member
Location: Bethesda MD Join Date: Oct 2009
Posts: 509
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It's entirely normal and does not affect the results.
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08-10-2020, 11:18 AM
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#3 |
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Junior Member
Location: Sevilla, Spain Join Date: Aug 2020
Posts: 2
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Okay, good to know - thanks for your answer!
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| Tags |
| fastqc, reads, wes, whole exome sequencing |
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