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Thread | Thread Starter | Forum | Replies | Last Post |
How to convert sra-lite format to fastq? | tbusch0000 | Bioinformatics | 23 | 08-21-2013 09:53 PM |
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#1 |
Member
Location: baltimore Join Date: Oct 2009
Posts: 89
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Dear group,
I have a question about .lite.sra files. For instance in a study there are 4 experiments (4 different SRX entries). In each SRX entry, there are different number of SRR.lite.sra files. for example SRX0001 there are 3 different SRR1.lite.SRA to SRR3.lite.SRA Are these 3 different runs (meaning 3 different fastq files) or you combine 3 lite.SRA files into one fastq file. What should be done. Please help. thanks Adrian |
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#2 |
Senior Member
Location: Massachusetts Join Date: May 2009
Posts: 116
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Can you provide the actual accession number? Answer may be different depending on whether the data are from a multiplexed run or not.
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#3 |
Senior Member
Location: Ohio Join Date: Jan 2010
Posts: 144
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In a data set I recently looked at, each SRR-lite file was from a different subject, and the SRX files appeared to be (454) instrument runs. So there were 5 SRXs and about 300 SRRs. I am not sure if that is a general phenomenon, but you hopefully can tell what is what by using the run browser.
It depends on what you want to do with the data. I didn't care about the individual subjects or runs and just wanted everything together. |
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