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01-12-2012, 06:05 PM
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Junior Member
Location: California Join Date: Jan 2012
Posts: 1
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HiC - crosslinking - glycine
hello. i have 2 questions.
1. what would be the best way to evaluate if the cross-linking went okay for Hi-C (chromosome interactions)? 2. i read different things about the temperature of quenching the formaldehyde with glycine. some do it at RT, some do it on ice or with ice-cold glycine. i am not a chemist. why would the temperature be of importance? thanks for reading the post and your kind help! Seb |
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| Tags |
| crosslinking, formaldehyde, glycine, hic |
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