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Old 02-16-2012, 02:58 AM   #1
Strand SI
The Avadis NGS Team
Location: All over the World

Join Date: Feb 2011
Posts: 26
Default Benchtop Sequencing Data Analysis - Webinar Series

We are hosting an online seminar series on the alignment and analysis of genomics data from “benchtop” sequencers, i.e. MiSeq and Ion Torrent. Our webinar panelists will give a tour of various bioinformatics functions in Avadis NGS that will enable researchers and clinicians to derive biological insights from their benchtop sequencing data.

Seminar #1: MiSeq Data Analysis

Avadis NGS 1.3 provides special support for analyzing data generated by MiSeq™ sequencers. In this webinar, we will describe how the data in a MiSeq generated “run folder” is automatically loaded into the Avadis NGS software during small RNA alignment and DNA variant analysis. This is especially helpful in processing the large number of files generated when the TruSeq™ Amplicon Kits are used. We will describe how to use the Quality Control steps in Avadis NGS to check if the amplicons have sufficient coverage in all the samples. Regions with unexpected coverages can easily be identified using the new region list clustering feature. Webinar attendees will learn how to use the “Find Significant SNPs” feature to quickly identify high-confidence SNPs present in a majority of the samples, rare variants, etc.

Seminar #2: Ion Torrent Data Analysis

Avadis NGS 1.3 includes a new aligner – COBWeb – that is fully capable of aligning the long, variable-length reads generated by Ion Torrent sequencers. In this webinar, we will show the pre-alignment QC plots and illustrate how they can be used to set appropriate alignment parameters for aligning Ion Torrent reads. For users who choose to import the BAM format files generated by the Ion Torrent Server, we will describe the steps needed for importing amplicon sequencing data into Avadis NGS. Users of the Ion AmpliSeq™ Cancer Panel will learn how to easily import the targeted mutation list and verify the genotype call at the mutation sites. We will also show the new “Find Significant SNPs” feature which helps quickly identify high-confidence SNPs present in a majority of the samples, rare variants, etc.

Free registration -
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alignment, bioinformatics, data analysis, ion torrent, miseq

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